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hek293 human embryonic kidney cell line  (ATCC)


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    ATCC hek293 human embryonic kidney cell line
    Hek293 Human Embryonic Kidney Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+kidney+cell+lines+hek293/HEK+293+STF%3B+Embryonic+Kidney%3B+Human/pm41683879-440-1-11
    Average 95 stars, based on 85 article reviews
    hek293 human embryonic kidney cell line - by Bioz Stars, 2026-10
    95/100 stars

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    Cell Culture:

    Article Title: In Vitro Anti-Inflammatory and Anticancer Evaluation of Mentha spicata L. and Matricaria chamomilla L. Essential Oils
    Article Snippet: .. Human breast cancer cell lines MCF7 (ATCC HTB-22), human lung cancer cell lines A549 (ATCC CCL-185), human prostate cancer cell lines PC3 (ATCC CRL-1435), and human embryonic kidney cell lines HEK293 (ATCC CRL-1573) were from American Type Culture Collection and were cultured in Dulbecco’s modified Eagle’s medium/high glucose (DMEM, high glucose) or Dulbecco’s modified Eagle’s medium/F12 (DMEM, F12) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% (v/v) antibiotic–antimycotics solution (100 U/mL penicillin,100 μg/mL streptomycin, and 0.25 μg/mL amphotericin B), and 1% (v/v) nonessential amino acids at 37 °C in a humidified 5% CO 2 atmosphere. ..

    Article Title: S-phase arrest and apoptosis in human breast adenocarcinoma MCF-7 cells via mitochondrial dependent pathway induced by tricyclohexylphosphine gold (I) n-mercaptobenzoate complexes.
    Article Snippet: We have previously reported the inhibition of thioredoxin reductase (TrxR) and invasion by tricyclohexylphosphine gold (I) n-mercaptobenzoate (n = 2, 3, 4) labeled as 1–3 towards MCF-7 cells, in vitro.. Nevertheless, the mode of death and its apoptotic pathway has yet to be revealed.. The main aim of this study is to investigate the anti-neoplastic activity of this phosphanegold (I) thiolates against breast adenocarcinoma cells, MCF-7.

    Article Title: Avian influenza viruses suppress innate immunity by inducing trans-transcriptional readthrough via SSU72.
    Article Snippet: All animal studies were conducted following the National Guidelines for the Care of Laboratory Animals and performed in accordance with institutional regulations and with the approval of experimental protocols by the Institutional Animal Care and Use Committee of the Institute of Military Veterinary, Academy of Military Medical Sciences. .. Cell lines and cell culture The human lung adenocarcinoma cell lines A549 and H1650, human cervical adenocarcinoma cell line HeLa, and human embryonic kidney cell lines HEK293 and HEK293T were purchased from the American Type Culture Collection (ATCC; Rockville, MD, USA). .. Adult human pulmonary fibroblast (HPF-a) cells were purchased from ScienCell Research Laboratories (ScienCell Research Laboratories, Inc., Carlsbad, CA, USA).

    Modification:

    Article Title: In Vitro Anti-Inflammatory and Anticancer Evaluation of Mentha spicata L. and Matricaria chamomilla L. Essential Oils
    Article Snippet: .. Human breast cancer cell lines MCF7 (ATCC HTB-22), human lung cancer cell lines A549 (ATCC CCL-185), human prostate cancer cell lines PC3 (ATCC CRL-1435), and human embryonic kidney cell lines HEK293 (ATCC CRL-1573) were from American Type Culture Collection and were cultured in Dulbecco’s modified Eagle’s medium/high glucose (DMEM, high glucose) or Dulbecco’s modified Eagle’s medium/F12 (DMEM, F12) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% (v/v) antibiotic–antimycotics solution (100 U/mL penicillin,100 μg/mL streptomycin, and 0.25 μg/mL amphotericin B), and 1% (v/v) nonessential amino acids at 37 °C in a humidified 5% CO 2 atmosphere. ..



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    Mechanistic association between QRICH1 and USP1 or Snail1 in Huh7 and SK-Hep-1 cells through their binding. (A) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. ***p<0.001 vs untreated control. (B) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (C) Interaction between QRICH1 and USP1 in <t>HEK293</t> cells by Flag IP and IB. (D) The direct binding between QRICH1 and USP1 in Huh7 and SK-Hep-1 cells by IP. (E) Effect of USP1 depletion on QRICH1 and Snail1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. **p<0.01, ***p<0.001 vs untreated control. (F) Effect of USP1 depletion on QRICH1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (G) Effect of QRICH1 depletion and USP1 overexpression on USP1 and Snail1 in Huh7 and SK-Hep-1 cells. (H) Effect of USP1 depletion on the binding to QRICH1 and Sanil1 in Huh7 and SK-Hep-1 cells.
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    The cell line name is indicated on the left side of each expression track.

    Journal: Life Science Alliance

    Article Title: The noncoding RNA GRAS1 interacts with NKAP and protects against DNA damage and cell death

    doi: 10.26508/lsa.202403105

    Figure Lengend Snippet: The cell line name is indicated on the left side of each expression track.

    Article Snippet: Human lung adenocarcinoma A549 cell line (ATCC No. CCL-185), human colorectal carcinoma HCT116 cell line (ATCC No. CCL-247), and human kidney embryonic HEK293 cell line (ATCC No. CRL-1573) were purchased from ATCC.

    Techniques: Expressing

    The cell line and replicate are indicated on the left side of each expression track.

    Journal: Life Science Alliance

    Article Title: The noncoding RNA GRAS1 interacts with NKAP and protects against DNA damage and cell death

    doi: 10.26508/lsa.202403105

    Figure Lengend Snippet: The cell line and replicate are indicated on the left side of each expression track.

    Article Snippet: Human lung adenocarcinoma A549 cell line (ATCC No. CCL-185), human colorectal carcinoma HCT116 cell line (ATCC No. CCL-247), and human kidney embryonic HEK293 cell line (ATCC No. CRL-1573) were purchased from ATCC.

    Techniques: Expressing

    (A) CCK8 assay for survival of A549, HCT116, and HEK293 cells transfected with control GapmeR compared with GRAS1 GapmeR1 or GRAS1 GapmeR2; N = 3. (B) CCK8 assay for survival of A549 and HCT116 cells transfected with empty vector compared with SNORA13 vector; N = 4. (C) CCK8 assay for survival of A549 and HCT116 cells transfected with empty vector compared with TIGA1 vector; N = 4. (D) Schematic gene structure of GRAS1 GapmeR–resistant construct. (E) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with GRAS1 GapmeR–resistant vector compared with empty vector; N = 4. (F) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with SNORA13 vector compared with empty vector; N = 4. (G) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with TIGA1 vector compared with empty vector; N = 4. Error bars represent SD in all panels; (ns) P > 0.05; (*) P ≤ 0.05; (**) P ≤ 0.01; (***) P < 0.001 by two-tailed t test.

    Journal: Life Science Alliance

    Article Title: The noncoding RNA GRAS1 interacts with NKAP and protects against DNA damage and cell death

    doi: 10.26508/lsa.202403105

    Figure Lengend Snippet: (A) CCK8 assay for survival of A549, HCT116, and HEK293 cells transfected with control GapmeR compared with GRAS1 GapmeR1 or GRAS1 GapmeR2; N = 3. (B) CCK8 assay for survival of A549 and HCT116 cells transfected with empty vector compared with SNORA13 vector; N = 4. (C) CCK8 assay for survival of A549 and HCT116 cells transfected with empty vector compared with TIGA1 vector; N = 4. (D) Schematic gene structure of GRAS1 GapmeR–resistant construct. (E) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with GRAS1 GapmeR–resistant vector compared with empty vector; N = 4. (F) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with SNORA13 vector compared with empty vector; N = 4. (G) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with TIGA1 vector compared with empty vector; N = 4. Error bars represent SD in all panels; (ns) P > 0.05; (*) P ≤ 0.05; (**) P ≤ 0.01; (***) P < 0.001 by two-tailed t test.

    Article Snippet: Human lung adenocarcinoma A549 cell line (ATCC No. CCL-185), human colorectal carcinoma HCT116 cell line (ATCC No. CCL-247), and human kidney embryonic HEK293 cell line (ATCC No. CRL-1573) were purchased from ATCC.

    Techniques: CCK-8 Assay, Transfection, Control, Plasmid Preparation, Construct, Knockdown, Two Tailed Test

    Cell viability of SH-SY5Y and  HEK293  cells treated with the compounds for 24 h. Data are mean ± SD ( n = 3)

    Journal: RSC Medicinal Chemistry

    Article Title: Design and synthesis of carbazole–acrylamide–pyridinium hybrids as dual acetylcholinesterase/butyrylcholinesterase inhibitors and evaluation of their cytotoxic profile on SH-SY5Y and HEK293 cell lines

    doi: 10.1039/d5md01075f

    Figure Lengend Snippet: Cell viability of SH-SY5Y and HEK293 cells treated with the compounds for 24 h. Data are mean ± SD ( n = 3)

    Article Snippet: The human neuroblastoma cell line SH-SY5Y (ATCC CRL-2266) and the human embryonic kidney cell line HEK293 (ATCC CRL-1573) were cultured in DMEM/F-12 supplemented with 10% heat-inactivated FBS and 1% penicillin–streptomycin solution.

    Techniques:

    Mechanistic association between QRICH1 and USP1 or Snail1 in Huh7 and SK-Hep-1 cells through their binding. (A) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. ***p<0.001 vs untreated control. (B) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (C) Interaction between QRICH1 and USP1 in HEK293 cells by Flag IP and IB. (D) The direct binding between QRICH1 and USP1 in Huh7 and SK-Hep-1 cells by IP. (E) Effect of USP1 depletion on QRICH1 and Snail1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. **p<0.01, ***p<0.001 vs untreated control. (F) Effect of USP1 depletion on QRICH1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (G) Effect of QRICH1 depletion and USP1 overexpression on USP1 and Snail1 in Huh7 and SK-Hep-1 cells. (H) Effect of USP1 depletion on the binding to QRICH1 and Sanil1 in Huh7 and SK-Hep-1 cells.

    Journal: International Journal of Biological Sciences

    Article Title: Targeting QRICH1 suppresses epithelial-mesenchymal transition and tumor growth in liver cancer through the Connexin43/USP1 mediated Snail1 stabilization

    doi: 10.7150/ijbs.133080

    Figure Lengend Snippet: Mechanistic association between QRICH1 and USP1 or Snail1 in Huh7 and SK-Hep-1 cells through their binding. (A) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. ***p<0.001 vs untreated control. (B) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (C) Interaction between QRICH1 and USP1 in HEK293 cells by Flag IP and IB. (D) The direct binding between QRICH1 and USP1 in Huh7 and SK-Hep-1 cells by IP. (E) Effect of USP1 depletion on QRICH1 and Snail1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. **p<0.01, ***p<0.001 vs untreated control. (F) Effect of USP1 depletion on QRICH1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (G) Effect of QRICH1 depletion and USP1 overexpression on USP1 and Snail1 in Huh7 and SK-Hep-1 cells. (H) Effect of USP1 depletion on the binding to QRICH1 and Sanil1 in Huh7 and SK-Hep-1 cells.

    Article Snippet: The liver cancer cell lines Huh7, SK-Hep-1, HepG2, SNU-449 and human embryonic kidney cell line HEK293 were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea).

    Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Control, Western Blot, Over Expression

    USP1 dependent regulation of Snail1 ubiquitination and specification of ubiquitin chain linkages. (A) Detection of Snail1 ubiquitination in Huh7 and SK-Hep-1 cells transfected with siQRICH1 and treated with MG132. (B) Assessment of ubiquitinated Snail1 in SNU-449 and HEK293 cells coexpressing the indicated vectors following a 4 h MG132 treatment, using Flag IP and subsequent IB analysis. (C) Determination of Snail1 ubiquitin-chain linkage patterns in HEK293 cells cotransfected with Flag-Snail1, Myc-USP1, and the designated His-Ub variants (WT, K48-only or K63-only). (D) Validation of ubiquitin-linkage in HEK293 cells expressing Flag-Snail1, Myc-USP1, and the indicated His-Ub plasmid (K6, K11, K27, K29, K33).

    Journal: International Journal of Biological Sciences

    Article Title: Targeting QRICH1 suppresses epithelial-mesenchymal transition and tumor growth in liver cancer through the Connexin43/USP1 mediated Snail1 stabilization

    doi: 10.7150/ijbs.133080

    Figure Lengend Snippet: USP1 dependent regulation of Snail1 ubiquitination and specification of ubiquitin chain linkages. (A) Detection of Snail1 ubiquitination in Huh7 and SK-Hep-1 cells transfected with siQRICH1 and treated with MG132. (B) Assessment of ubiquitinated Snail1 in SNU-449 and HEK293 cells coexpressing the indicated vectors following a 4 h MG132 treatment, using Flag IP and subsequent IB analysis. (C) Determination of Snail1 ubiquitin-chain linkage patterns in HEK293 cells cotransfected with Flag-Snail1, Myc-USP1, and the designated His-Ub variants (WT, K48-only or K63-only). (D) Validation of ubiquitin-linkage in HEK293 cells expressing Flag-Snail1, Myc-USP1, and the indicated His-Ub plasmid (K6, K11, K27, K29, K33).

    Article Snippet: The liver cancer cell lines Huh7, SK-Hep-1, HepG2, SNU-449 and human embryonic kidney cell line HEK293 were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea).

    Techniques: Ubiquitin Proteomics, Transfection, Biomarker Discovery, Expressing, Plasmid Preparation

    Effect of QRICH1 overexpression on proliferation, migration and EMT and cytoskeleton related proteins in SNU-449 and HEK293 cells (A) QRICH1, USP1, and Snail1 mRNA levels in SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. ***p < 0.001 vs. untreated control. (B) Clonogenic capacity of SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. **p<0.01, ***p<0.001 vs untreated control. (C) Migratory capacity of SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. Scale bar, 200 μm. *p<0.05 vs untreated control. (D) EMT- and cytoskeleton-related protein profiles of SNU-449 and HEK293 cells with QRICH1 overexpression. *p<0.05, **p<0.01, ***p<0.001 vs untreated control.

    Journal: International Journal of Biological Sciences

    Article Title: Targeting QRICH1 suppresses epithelial-mesenchymal transition and tumor growth in liver cancer through the Connexin43/USP1 mediated Snail1 stabilization

    doi: 10.7150/ijbs.133080

    Figure Lengend Snippet: Effect of QRICH1 overexpression on proliferation, migration and EMT and cytoskeleton related proteins in SNU-449 and HEK293 cells (A) QRICH1, USP1, and Snail1 mRNA levels in SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. ***p < 0.001 vs. untreated control. (B) Clonogenic capacity of SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. **p<0.01, ***p<0.001 vs untreated control. (C) Migratory capacity of SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. Scale bar, 200 μm. *p<0.05 vs untreated control. (D) EMT- and cytoskeleton-related protein profiles of SNU-449 and HEK293 cells with QRICH1 overexpression. *p<0.05, **p<0.01, ***p<0.001 vs untreated control.

    Article Snippet: The liver cancer cell lines Huh7, SK-Hep-1, HepG2, SNU-449 and human embryonic kidney cell line HEK293 were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea).

    Techniques: Over Expression, Migration, Transfection, Plasmid Preparation, Control